![]() For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, DF V i V f 1mL 10mL 1 10. ![]() The dilution factor or the dilution is the initial volume divided by the final volume. The highly concentrated solution is typically referred to as the stock solution. In serial dilutions, you multiply the dilution factors for each step. Consequently, the molarity is one-fifth of its original value.Īnother common dilution problem involves calculating what amount of a highly concentrated solution is required to make a desired quantity of solution of lesser concentration. A serial dilution is a series of dilutions made sequentially, using the same dilution factor for each step. One of the most common series doubles the dilution factor with each transfer (1:2, 1:4, 1:8. A small amount of serum or solute can be serially diluted by transferring aliquots to diluent. Serial dilutions are also commonly used to avoid having to pipette very small volumes (1-10 µl) to make a dilution of a solution. Serial dilution is a common technique used in many immunologic procedures. ![]() For example, if A 280 says you have 7.0 mg total protein/ml, and you think the protein could be anywhere between 10 and 100 pure, then your assay needs to be able to see anything between 0.7 and 7 mg/ml. Serial two-fold and ten-fold dilutions are commonly used to titer antibodies or prepare diluted analytes (for a standard curve for example). 2 for the specific serial dilution experiment (i.e., 10, p 1, d plate 10cm), for other serial dilution parameters the figure RE n must be redrawn (since k is a function of, p, d colony, d plate see Eq. The solution has been diluted by one-fifth since the new volume is five times as great as the original volume. Before making serial dilutions, you need to make rough estimates of the concentrations in your unknowns, and your uncertainty in those estimates. This range can be obtained pictorially from Fig. ![]()
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